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Molecular Diversity Among Members of the Saccharum Complex Assessed Using TRAP Markers Based on Lignin-Related Genes
- Suman, Andru, Ali, Kazim, Arro, Jie, Parco, Arnold S., Kimbeng, Collins A., Baisakh, Niranjan
- BioEnergy research 2012 v.5 no.1 pp. 197-205
- Miscanthus, Saccharum barberi, Saccharum officinarum, Saccharum robustum, Saccharum sinense, Saccharum spontaneum, alcohol dehydrogenase, biomass production, biotransformation, caffeate O-methyltransferase, cultivars, ethanol, genes, genetic relationships, genetic variation, genotype, grasses, lignin, sugarcane, sugars
- In addition to the cultivation of sugarcane for sugar, the crop is considered seriously as an important bioenergy grass crop for its high biomass production ability. But, lignin is a serious bottleneck in the bioconversion of lignocellulosic biomass to ethanol. Hence, genetic relationships among 64 genotypes within the Saccharum complex were studied with respect to lignin-related genes using target region amplified polymorphic (TRAP) primers derived from caffeic acid O-methyltransferase (COMT), cinnamoyl alcohol dehydrogenase (CAD), cinnamoyl coA reductase (CCR), and ferrulate 5-hydroxylase (F5H) genes. While the average polymorphism detected by the TRAP markers was 43%, the markers derived from F5H gene (34%) were less polymorphic in comparison to those derived from COMT (46%), CCR (44%), and CAD (46%) genes. The lignin gene-based TRAP markers differentiated members of the Saccharum complex broadly according to previously established genetic relationships in the order of Miscanthus > Erianthus > Saccharum spontaneum > Saccharum robustum/Saccharum barberi/Saccharum sinense > Saccharum officinarum/cultivars. Principal coordinate analysis showed that 29% of the total variation was explained by the genotypes with respect to the lignin-related genes. The association of genetic variation revealed in this study with the biomass composition-related genes of the genotypes within a species will be helpful to design breeding strategies to develop superior energy cane cultivars with improved biomass quality of the sugarcane.