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Proteomic Analysis of Phloem Proteins Leads to the Identification of Potential Candidates for JA-Mediated RKN-Resistant Elements in Solanum lycopersicum

Zhao, Wenchao, Hao, Jinghong, Xing, Jiayi, Yang, Rui, Zhao, Fukuan, Wang, Jianli, Wang, Shaohui
Journal of plant growth regulation 2017 v.36 no.1 pp. 96-105
Meloidogyne, Solanum lycopersicum, crop production, energy conversion, financial economics, gels, jasmonic acid, matrix-assisted laser desorption-ionization mass spectrometry, messenger RNA, phloem, photosynthesis, protein synthesis, proteins, proteome, proteomics, quantitative polymerase chain reaction, reverse transcriptase polymerase chain reaction, root-knot nematodes, tomatoes, two-dimensional gel electrophoresis
Tomato production has been severely affected by root-knot nematode (RKN) diseases, leading to huge economic losses in tomato cultivation, production, and processing. To gain insight into the signal mechanism of resistance to RKN (Meloidogyne incongnita), tomato lines with different endogenous jasmonic acid levels were inoculated with RKN, and the differential proteome of their phloem was analyzed with two-dimensional gel electrophoresis. Analysis of 1400 protein spots from each gel revealed 74 differentially expressed proteins, 30 of which were identifed via matrix-assisted laser-desorption ionization time-of-flight mass spectrometry (MALDI-TOF–MS). Among these 30, the abundance of 25 proteins was elevated in 35S::PS, and five proteins were not expressed in spr2. The total differentially expressed proteins were grouped into multiple functional categories, the largest of which was energy conversion (38 %). Furthermore, we proposed several candidates that might function as potential signal molecules under RKN stress and dissected the multiple roles of proteins related to photosynthesis and energy conversion. The mRNA levels of nine proteins associated with defense responses and energy metabolism were analyzed by qRT-PCR, and the expression levels of five were in line with the proteome data.