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Selenium Improves Physiological Parameters and Alleviates Oxidative Stress in Shoots of Lead-Exposed Vicia faba L. minor Plants Grown Under Phosphorus-Deficient Conditions

Author:
Mroczek-Zdyrska, Magdalena, Strubińska, Joanna, Hanaka, Agnieszka
Source:
Journal of plant growth regulation 2017 v.36 no.1 pp. 186-199
ISSN:
0721-7595
Subject:
Vicia faba, carotenoids, catalase, chlorophyll, faba beans, glutathione peroxidase, hydrogen peroxide, lead, leaves, lipid peroxidation, nutrient solutions, oxidative stress, peroxidase, selenites, selenium, shoots, tissues, toxicity
Abstract:
The goal of the study was to investigate the effects of exogenous selenium (Se) on the tolerance of faba bean plants to lead (Pb) stress under P-deficient conditions. The bean plants were grown for 2 weeks on Hoagland solution supplied with Pb (0, 50 μM) and Se (0, 1.5, or 6 μM), separately or simultaneously. It was shown that Pb did not affect shoot growth but caused major damage in the leaves, which was accompanied by Pb accumulation in these tissues. The exposure of the shoots to Pb led to significant changes in the biochemical parameters: the MDA content, glutathione peroxidase (GSH-Px), guaiacol peroxidase (GPOX), and catalase (CAT) activity increased. Furthermore, Pb intensified O ₂ •⁻ and H₂O₂ production. Both the Se concentrations used increased the chlorophyll b, chlorophyll a+b, and carotenoid content in the faba bean plants. Selenite also generally enhanced CAT, GPOX, and GSH-Px activities and the T-SH level. Our results imply that the degree of disturbances caused by Pb could be partially ameliorated by Se supplementation. Selenite at a lower dose alleviated Pb toxicity by decreased H₂O₂ and O ₂ •⁻ production and decreased the GSH-Px, GPOX, and CAT activities. The beneficial effect of the higher selenite concentration could be related to reduction of lipid peroxidation in the shoots of the Pb-treated plants. However, the effect of Se on the Pb-stressed plants greatly depended on the selenite dose in the nutrient solution.
Agid:
5759198