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Direct Characterization of Native Chemical Ligation of Peptides on Silicon Nanowires

Author:
Dendane, Nabil, Melnyk, Oleg, Xu, Tao, Grandidier, Bruno, Boukherroub, Rabah, Stiévenard, Didier, Coffinier, Yannick
Source:
Langmuir 2012 v.28 no.37 pp. 13336-13344
ISSN:
1520-5827
Subject:
X-ray photoelectron spectroscopy, chemoselectivity, cysteine, moieties, nanowires, peptides, silicon
Abstract:
We describe the site-specific and chemoselective immobilization of peptides on hydrogen-terminated silicon nanowires (SiNWs) using native chemical ligation (NCL) (i.e., the reaction of a thioester group with a cysteine moiety to give a stable amide bond). The SiNWs investigated in this work were grown via a vapor–liquid–solid mechanism and functionalized with a thioester moiety. The immobilization of the peptides on the SiNWs was demonstrated by synthesizing peptides with an N-terminal cysteine residue and labeled with tetramethylrhodamine or trifluoromethyl groups that were detected by fluorescence and X-ray photoelectron spectroscopy, respectively. The peptides labeled with tetramethylrhodamine or trifluoromethyl groups for fluorescence or X-ray photoelectron spectroscopy (XPS) detection studies were synthesized with an N-terminal cysteine residue. N-Terminal seryl peptides and carboxy-terminated SiNWs were used as controls to demonstrate the chemoselectivity of the peptide immobilization.
Agid:
5766417