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Scientific note on mass collection and hatching of honey bee embryos
- Evans, Jay D., Boncristiani Jr., Humberto, Chen, Yanping
- Apidologie 2010 v.41 no.6 pp. 654
- Apis mellifera, adults, bee diseases, diagnostic techniques, embryo (animal), entomopathogens, hatching, honey bee colonies, parasites, screening, surveys, worker honey bees
- Honey bees provide excellent opportunities for studying development, behavior, and defenses against natural parasites and pathogens. They are also a critical component of modern agriculture, through their leading role in pollinating the world’s crops (Aizen and Harder, 2009). Research and diagnostic methods for honey bees require efficient sampling methods for all colony members, often involving a large number of individual bees. As one example, surveys of honey bees for their pathogens require substantial sample sizes within colonies, especially when there is variation across individuals in infection rate or pathogen loads. This need for greater sampling must be balanced by the expenses involved with collecting and screening numerous colony members. In the case of whole-colony surveys for pathogens, mass extractions from adult workers require scaled up amounts of expensive reagents and, as a result, most such surveys have been limited to a small subset of colony members (vanEngelsdorp et al., 2009).