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Development of a core set of SSR markers for the characterization of Gossypium germplasm

Yu, John Z., Fang, David D., Kohel, Russell J., Ulloa, Mauricio, Hinze, Lori L., Percy, Richard G., Zhang, Jinfa, Chee, Peng, Scheffler, Brian E., Jones, Don C.
Euphytica 2012 v.187 no.2 pp. 203-213
DNA, DNA fingerprinting, Gossypium hirsutum, chromosomes, cotton, databases, fluorescent dyes, genetic markers, genetic variation, genotype, germplasm, microsatellite repeats, phylogeny, research projects
Molecular markers such as simple sequence repeats (SSR) are a useful tool for characterizing genetic diversity of Gossypium germplasm. Genetic profiles by DNA fingerprinting of cotton accessions can only be compared among different collections if a common set of molecular markers are used by different laboratories and/or research projects. Herein, we propose and report a core set of 105 SSR markers with wide genome coverage of at least four evenly distributed markers per chromosome for the 26 tetraploid cotton chromosomes. The core marker set represents the efforts of ten research groups involved in marker development, and have been systematically evaluated for DNA polymorphism on the 12 genotypes belonging to six Gossypium species [known collectively as the cotton marker database (CMD) panel]. A total of 35 marker bins in triplex sets were arranged from the 105 markers that were each labeled with one of the three fluorescent dyes (FAM, HEX, and NED). Results from this study indicated that the core marker set was robust in revealing DNA polymorphism either between and within species. Average value of polymorphism information content (PIC) among the CMD panel was 0.65, and that within the cultivated cotton species Gossypium hirsutum was 0.29. Based on the similarity matrix and phylogenetic analysis of the CMD panel, the core marker set appeared to be sufficient in characterizing the diversity within G. hirsutum and other Gossypium species. The portability of this core marker set would facilitate the systematic characterization and the simultaneous comparison among various research efforts involved in genetic diversity analysis and germplasm resource preservation.