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Secretion and Reversible Assembly of Extracellular-like Matrix by Enzyme-Active Colloidosome-Based Protocells

Author:
Akkarachaneeyakorn, Khrongkhwan, Li, Mei, Davis, Sean A., Mann, Stephen
Source:
Langmuir 2016 v.32 no.12 pp. 2912-2919
ISSN:
1520-5827
Subject:
dephosphorylation, hydrogels, secretion
Abstract:
The secretion and reversible assembly of an extracellular-like matrix by enzyme-active inorganic protocells (colloidosomes) is described. Addition of N-fluorenyl-methoxycarbonyl-tyrosine-(O)-phosphate to an aqueous suspension of alkaline phosphatase-containing colloidosomes results in molecular uptake and dephosphorylation to produce a time-dependent sequence of supramolecular hydrogel motifs (outer membrane wall, cytoskeletal-like interior and extra-protocellular matrix) that are integrated and remodelled within the microcapsule architecture and surrounding environment. Heat-induced disassembly of the extra-protocellular matrix followed by cooling produces colloidosomes with a densely packed hydrogel interior. These procedures are exploited for the fabrication of nested colloidosomes with spatially delineated regions of hydrogelation.
Agid:
5773906