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stageR: a general stage-wise method for controlling the gene-level false discovery rate in differential expression and differential transcript usage
- Van den Berge, Koen, Soneson, Charlotte, Robinson, Mark D., Clement, Lieven
- Genome biology 2017 v.18 no.1 pp. 151
- gene expression regulation, genes, sequence analysis
- RNA sequencing studies with complex designs and transcript-resolution analyses involve multiple hypotheses per gene; however, conventional approaches fail to control the false discovery rate (FDR) at gene level. We propose stageR, a two-stage testing paradigm that leverages the increased power of aggregated gene-level tests and allows post hoc assessment for significant genes. This method provides gene-level FDR control and boosts power for testing interaction effects. In transcript-level analysis, it provides a framework that performs powerful gene-level tests while maintaining biological interpretation at transcript-level resolution. The procedure is applicable whenever individual hypotheses can be aggregated, providing a unified framework for complex high-throughput experiments.