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Chemical Composition and Antibacterial Action of Robinia pseudoacacia L. Flower Essential Oil on Membrane Permeability of Foodborne Pathogens

Author:
Bhalla, Piyush, Bajpai, Vivek K.
Source:
Journal of essential oil-bearing plants 2017 v.20 no.3 pp. 632-645
ISSN:
0976-5026
Subject:
Bacillus subtilis, Escherichia coli O157, Listeria monocytogenes, Robinia pseudoacacia, Salmonella enterica, Staphylococcus aureus, adenosine triphosphate, antimicrobial properties, cations, chemical composition, diameter, essential oils, flowers, food pathogens, gas chromatography-mass spectrometry, membrane permeability, oils, potassium
Abstract:
This research investigates the chemical composition of flower essential oil of Robinia pseudoacacia (FEORP) by GC-MS analysis and reports its antimicrobial efficacy against selected foodborne pathogens. The GC-MS analysis of FEORP confirmed the presence of 27 compounds which represented 94.46 % of total oil. Further, FEORP (1000 μg/disc) exhibited significant antimicrobial effects against some of the selected foodborne pathogens such as Staphylococcus aureus KCTC 1621, Bacillus subtilis KCTC 3569, Listeria monocytogenes KCTC 3569, Escherichia coli O157:H7 and Salmonella enterica ATCC 4731 with diameters of the zones of inhibition (15.2 ± 0.3-17.3 ± 2.0 mm) as well as the MIC and MBC (250-1,000 μg/mL). Additionally, FEORP at MIC reduced cell viabilities of two selected foodborne pathogens, B. subtilis KCTC 3569 and E. coli O157:H7. Furthermore, FEORP established its inhibitory effect on the membrane integrity of both selected bacterial pathogens as indicated by the increased release of extracellular ATP and relative electrical conductivities as well as the loss of 260-nm absorbing materials, and leakage of potassium ions, suggesting that FEORP could be an effective and natural agent for controlling the growth of hazardous foodborne pathogens.
Agid:
5776010