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Agrobacterium deltaense sp. nov., an endophytic bacteria isolated from nodule of Sesbania cannabina

Author:
Yan, Jun, Li, Yan, Han, Xiao Zeng, Chen, Wen Feng, Zou, Wen Xiu, Xie, Zhihong, Li, Meng
Source:
Archives of microbiology 2017 v.199 no.7 pp. 1003-1009
ISSN:
0302-8933
Subject:
Agrobacterium radiobacter, Sesbania cannabina, aldehydes, alpha-ketoglutaric acid, bacteria, carbon, citric acid, endophytes, essential genes, fatty acids, lactulose, nucleotide sequences, phenotype, phylogeny, polysorbates, ribosomal RNA, river deltas, root nodules, sequence homology, China, Yellow River
Abstract:
A Gram-negative, non-spore-forming, aerobic rods, strain YIC4121ᵀ, was isolated from root nodule of Sesbania cannabina grown in Dongying (Yellow River Delta), Shandong Province, PR China. Based on phylogenetic analysis of 16 S rRNA gene sequences, strain YIC4121ᵀ was assigned to the genus Agrobacterium with 99.7, 99.3, 99.0, 98.8 and 98.7% sequence similarities to Agrobacterium radiobacter LMG140ᵀ, A. pusense NRCPB10ᵀ, A. arsenijevicii KFB 330ᵀ, A. nepotum 39/7ᵀ and A. larrymoorei ATCC51759ᵀ. Analysis of the concatenated housekeeping genes (recA-atpD-glnII), showed lower sequence similarities (≤94.6%) between strain YIC4121ᵀ and other recognized Agrobacterium species. Strain YIC4121ᵀ shared whole-genome average nucleotide identities (ANI) 87.94, 91.27 and 77.42%, with A. pusense NRCPB10ᵀ, A. radiobacter LMG140ᵀ and A. larrymoorei ATCC51759ᵀ. The predominant cellular fatty acids were C₁₉:₀ cyclo ω8c, summed feature 2 (C₁₂:₀ aldehyde/unknown 10.9525), summed feature 8 (C₁₈:₁ ω7c/C₁₈:₁ ω6c), C₁₆:₀ 3 OH and C₁₆:₀. The G + C content of strain YIC4121ᵀ was 59.80 mol%. Tween 80, lactulose, citric acid, α-ketoglutaric acid, glycyl-L-glutamic acid and 2, 3-butanediol could not be utilized as carbon source, distinguishing strain YIC4121ᵀ from the other related species. Based on the distinctly genetic and phenotypic dissimilarity, a novel species Agrobacterium deltaense sp. nov. is proposed with YIC4121ᵀ (=HAMBI 3654ᵀ = LMG 29283ᵀ) as the type strain.
Agid:
5776075