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Autoinduction of a genetic locus encoding putative acyltransferase in Corynebacterium glutamicum

Author:
Hee-Sung Shin, Yong-Jae Kim, In-Hwa Yoo, Heung-Shick Lee, Shouguang Jin, Un-Hwan Ha
Source:
Biotechnology letters 2011 v.33 no.1 pp. 97-102
ISSN:
0141-5492
Subject:
Corynebacterium ammoniagenes, Corynebacterium glutamicum, Pseudomonas aeruginosa, acyltransferases, bacteria, cell free system, gene expression, genes, loci
Abstract:
A genetic locus, encoding putative acyltransferase, was induced by autoinducers in Corynebacterium glutamicum. The autoinducers were maximally produced by the bacterium after 24 h culture. Those molecules are resistant to proteinase K treatment (300 μg ml−1) for 30 min at 37°C or at 121°C for 15 min, and remained stable after extensive storage at 4°C. Autoinducers in the cell-free culture fluids from Corynebacterium ammoniagenes and Pseudomonas aeruginosa also induced the expression of acyltransferase in C. glutamicum, suggesting possible cross-recognition of the autoinducers by C. glutamicum. C. glutamicum thus possesses an autoinduction system which secretes autoinducers during growth, triggering the expression of downstream genes, exemplified by the putative acyltransferase gene.
Agid:
578065