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Fast detection of Listeria monocytogenes through a nanohybrid quantum dot complex

Author:
Donoso, Wendy, Castro, Ricardo I., Guzmán, Luis, López-Cabaña, Zoraya, Nachtigall, Fabiane M., Santos, Leonardo S.
Source:
Analytical and bioanalytical chemistry 2017 v.409 no.22 pp. 5359-5371
ISSN:
1618-2642
Subject:
Listeria monocytogenes, at-risk population, bacteria, crystals, detection limit, fluorescence, fluorescence microscopy, food pathogens, lipoic acid, listeriosis, quantum dots, semiconductors
Abstract:
Listeria monocytogenes is a recognized foodborne pathogen that causes listeriosis in susceptible consumers. Currently, the detection systems for Listeria in food detect live and dead bacteria, being the viable microorganisms most relevant for their ability to cause sickness in the population at risk. For this reason, a new nanohybrid compound was developed for the optical detection of Listeria that was based on polyamidoamine dendrimers functionalized with an auxotrophic cofactor (lipoic acid), together with the coupling of fluorescent semiconductor crystals (quantum dots). The nanohybrid sensor has a detection limit for viable L. monocytogenes of 5.19 × 10³ colony-forming units per milliliter under epifluorescence microscopy. It was specific when used among other pathogens commonly found in food.
Agid:
5781549