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Isolation, characterization, and cross-amplification of microsatellite markers for the Petunia integrifolia (Solanaceae) complex

Author:
Kriedt, Raquel A., Ramos-Fregonezi, Aline M. C., Beheregaray, Luciano B., Bonatto, Sandro L., Freitas, Loreta B.
Source:
American journal of botany 2011 v.98 no.10 pp. e277
ISSN:
0002-9122
Subject:
Petunia integrifolia, genotyping, introgression, loci, microsatellite repeats, parents, population genetics, screening
Abstract:
• Premise of the study: Microsatellite markers were developed for Petunia integrifolia subsp. depauperata with an intent to clarify taxonomic questions on the P. integrifolia complex, and to identify a purple-flowered parent of P. hybrida. • METHODS: and Results: We characterized 11 microsatellite loci by screening primers developed using an SSR-enriched library. Genotyping of two populations resulted in eight polymorphic loci. Cross-species transferability was tested for other members of the P. integrifolia complex. • CONCLUSIONS: The development of these markers may contribute to population genetics studies in Petunia, and cross-amplification among related species could be a useful tool for research on hybridization and introgression.
Agid:
578594