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Nontransformed and Cancer Cells Can Utilize Different Endocytic Pathways To Internalize Dendritic Nanoparticle Variants: Implications on Nanocarrier Design
- Wong, Nelson
K. Y., Shenoi, Rajesh A., Abbina, Srinivas, Chafeeva, Irina, Kizhakkedathu, Jayachandran N., Khan, Mohamed K.
- Biomacromolecules 2017 v.18 no.8 pp. 2427-2438
- endocytosis, hydrophilicity, hydrophobicity, nanocarriers, nanoparticles, neoplasm cells, neoplasms, polyethylene glycol
- Three hyperbranched polyglycerol nanoparticle (HPG NP) variants were synthesized and fluorescently labeled for the study of their cellular interactions. The polymeric nanoparticle that contains a hydrophobic core and a hydrophilic HPG shell, HPG-C₁₀-HPG, is taken up faster by HT-29 cancer cells than nontransformed cells, while similar uptake rates are observed with both cell types for the nanoparticle HPG-C₁₀-PEG that contains a hydrophobic core and a polyethylene glycol shell. The nanoparticle HPG-104, containing neither the hydrophobic core nor the polyethylene glycol shell, is taken up faster by nontransformed cells than HT-29 cells. Importantly, cancer and normal cells can utilize different endocytic mechanisms for the internalization of these HPG NPs. Both HPG-C₁₀-HPG and HPG-C₁₀-PEG are taken up by HT-29 cells through clathrin-mediated endocytosis and macropinocytosis. Nontransformed cells, however, take up HPG-C₁₀-HPG and HPG-104 through macropinocytosis, while these cells utilize both clathrin-mediated endocytosis and macropinocytosis to internalize HPG-C₁₀-PEG.