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A Second-Generation Phosphohistidine Analog for Production of Phosphohistidine Antibodies
- Kee, Jung-Min, Oslund, Rob C., Couvillon, Anthony D., Muir, Tom W.
- Organic letters 2015 v.17 no.2 pp. 187-189
- antibodies, chemical structure, histidine, phosphorylation, post-translational modification
- Protein histidine phosphorylation plays a crucial role in cell signaling and central metabolism. However, its detailed functions remain elusive due to technical challenges in detecting and isolating proteins bearing phosphohistidine (pHis), a labile posttranslational modification (PTM). To address this issue, we previously developed the first pHis-specific antibodies using stable, synthetic triazole-based pHis analogs. A second-generation, pyrazole-based pHis analog that enabled the development of a pan-pHis antibody with much improved pHis specificity is now reported.