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Decolorization of Congo Red by Phanerochaete chrysosporium: the role of biosorption and biodegradation

Bosco, Francesca, Mollea, Chiara, Ruggeri, Bernardo
Environmental technology 2017 v.38 no.20 pp. 2581-2588
Phanerochaete chrysosporium, agar, biodegradation, biomass, biosorption, decolorization, environmental technology, in vitro studies, lignin peroxidase, malt extract, manganese peroxidase, mycelium, temperature
The degradation of Congo Red by means of Phanerochaete chrysosporium BKM-F-1767 is reported in this work. Solid and liquid cultures have been prepared to evaluate in viv o biodegradation as well as the role of biosorption phenomena on mycelium. Moreover, in vitro tests have been performed to define the influence of MnP on dye decolorization. P. chrysosporium , cultivated on Malt Extract Agar in the presence of Congo Red 0.005% (w/v), has shown good growth and the ability to decolorize the dye in the 25–39°C temperature range. It has also been cultivated in a low NMM liquid medium with the aforementioned dye concentration in immobilized stationary cultures inducted for Lignin Peroxidase (LiP) and Manganese Peroxidase (MnP) production. Congo Red was absorbed on the biomass and then decolorized (93% and 85% for the LiP and MnP cultures, respectively). The cultures with added Congo Red have shown a higher MnP synthesis rate than a control without the dye. The enzymatic degradation of Congo Red has also been investigated by means of the extracellular fluid for different MnP activities (0–300 IU/l); the decolorization percentage has been found to be clearly related to the enzyme concentration up to a value of about 200 IU/l.