Molecular mapping of four blast resistance genes using recombinant inbred lines of 93-11 and nipponbare
- Source:
- Journal of plant biology 2013 v.56 no. pp. 91-97
- ISSN:
- 1867-0725
- Subject:
- Magnaporthe oryzae, blast disease, chromosome mapping, chromosomes, disease resistance, genes, genetic markers, inbred lines, marker-assisted selection, microsatellite repeats, new variety, phenotypic variation, quantitative trait loci
- Abstract:
- Molecular mapping of new blast resistance genes is important for developing resistant rice cultivars using marker-assisted selection. In this study, 259 recombinant inbred lines (RILs) were developed from a cross between Nipponbare and 93-11, and were used to construct a 1165.8-cM linkage map with 131 polymorphic simple sequence repeat (SSR) markers. Four major quantitative trait loci (QTLs) for resistance to six isolates of Magnaporthe oryzae were identified: qPi93-1, qPi93-2, qPi93-3, and qPiN-1. For the three genes identified in 93-11, qPi93-1 is linked with SSR marker RM116 on the short arm of chromosome 11 and explains 33% of the phenotypic variation in resistance to isolate CHE86. qPi93-2 is linked with SSR marker RM224 on the long arm of chromosome 11 and accounts for 31% and 25% of the phenotypic variation in resistance to isolates 162-8B and ARB50, respectively. qPi93-3 is linked with SSR marker RM7102 on chromosome 12 and explains 16%, 53%, and 28% of the phenotypic variation in resistance to isolates CHE86, ARB52, and ARB94, respectively. QTL qPiN-1 from Nipponbare is associated with SSR marker RM302 on chromosome 1 and accounts for 34% of the phenotypic variation in resistance to isolate PO6-6. These new genes can be used to develop new varieties with blast resistance via marker-aided selection and to explore the molecular mechanism of rice blast resistance.
- Agid:
- 57990
- Handle:
- 10113/57990
- https://doi.org/10.1007/s12374-012-0462-7