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Polymeric proteins and their association with grain yield in hard red spring wheat lines.

Tsilo, Toi J., Hareland, Gary A., Ohm, Jae-Bom, Anderson, James A.
Euphytica 2013 v.194 pp. 187
Triticum aestivum, absorbance, breeding, endosperm, flour, grain protein, grain yield, hard red spring wheat, high performance liquid chromatography, molecular weight, protein content, sulfates
Development of high yielding wheat (Triticum aestivum L.) varieties with acceptable end-use quality is a major focus in breeding programs worldwide. Variations in molecular weight (Mw) distribution of endosperm proteins are known to influence end-use quality traits. This paper reports the relationship of the size-exclusion high performance liquid chromatography (SE-HPLC) profile of endosperm proteins with grain yield. Flour samples were previously analyzed for Mw distribution of sodium-dodecyl sulfate (SDS) buffer extractable and unextractable proteins using the SE-HPLC protocol. Correlations were calculated between grain yield and HPLC absorbance data obtained at 0.01-min retention time intervals. Although both SDS-extractable and unextractable proteins had positive correlations with grain protein content, only SDS-unextractable very high Mw polymeric proteins (UVHP) had no negative association with grain yield, while SDS-extractable fractions rich in low Mw polymeric proteins had a negative correlation (r = -0.41) with grain yield. These results suggest that in an effort to increase grain yield, breeding programs should target grain yield and also increase levels of UVHP and decrease SDS extractable polymeric proteins, thereby, maintaining acceptable bread-making quality.