PubAg

Main content area

Versatile Methodology for Glycosurfaces: Direct Ligation of Nonderivatized Reducing Saccharides to Poly(pentafluorophenyl acrylate) Grafted Surfaces via Hydrazide Conjugation

Author:
Chen, Li, Leman, Deborah, Williams, Caitlin R., Brooks, Karson, Krause, Duncan C., Locklin, Jason
Source:
Langmuir 2017 v.33 no.35 pp. 8821-8828
ISSN:
1520-5827
Subject:
Mycoplasma pneumoniae, bioactive properties, glycosylation, humans, hydrazides, hydrazine, lectins, pathogens, respiratory system, sugars
Abstract:
In this work, we report a convenient and versatile strategy for surface-grafted glycopolymer constructs with the goal of surface modification that controls the chemical presentation and grafting density of carbohydrate side chains. This approach employs a difunctional hydrazine linker, chemically modified to an active ester containing poly(pentafluorophenyl acrylate) grafted scaffold, to conjugate a variety of saccharides through the reducing end. The successive conjugation steps are carried out under mild conditions and yield high surface densities of sugars, as high as 4.8 nmol·cm–², capable of multivalency, with an intact structure and retained bioactivity. We also demonstrate that this glycosylated surface can bind specific lectins according to the structure of its pendant carbohydrate. To demonstrate bioactivity, this surface platform is used to study the binding events of a human respiratory tract pathogen, Mycoplasma pneumoniae, on surfaces conjugated with sialylated sugars.
Agid:
5799442