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Disposable electrochemical immunosensor for Brettanomyces bruxellensis based on nanogold-reduced graphene oxide hybrid nanomaterial

Borisova, Boryana, Villalonga, MaríaL., Arévalo-Villena, María, Boujakhrout, Abderrahmane, Sánchez, Alfredo, Parrado, Concepción, Pingarrón, JoséM., Briones-Pérez, Ana, Villalonga, Reynaldo
Analytical and bioanalytical chemistry 2017 v.409 no.24 pp. 5667-5674
3-mercaptopropionic acid, antibodies, carbon, electrochemistry, electrodes, gold, graphene oxide, immunosensors, nanomaterials, red wines, spoilage, storage quality, yeasts
The assembly of a novel disposable amperometric immunosensor for the detection of the red wine spoilage yeast Brettanomyces bruxellensis is reported. The nanostructured sensing interface was prepared by first coating carbon screen printed electrodes with a gold nanoparticles-reduced graphene oxide hybrid nanomaterial, which was then modified with 3-mercaptopropionic acid to further immobilize specific antibodies for B. bruxellensis via a carbodiimide-coupling reaction. The functionalized electrode allowed the amperometric detection of B. bruxellensis in buffered solutions and red wine samples in the range of 10–10⁶ CFU/mL and 10²–10⁶ CFU/mL, with low detection limits of 8 CFU/mL and 56 CFU/mL, respectively. The electrochemical immunosensor also exhibited high reproducibility, selectivity, and storage stability. Graphical abstract A novel disposable electrochemical immunosensor for the detection of the red wine spoilage yeast B. bruxellensis