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Comparison of physicochemical pretreatments of banana peels for bioethanol production
- Palacios, Sócrates, Ruiz, Héctor A., Ramos-Gonzalez, Rodolfo, Martínez, José, Segura, Elda, Aguilar, Miguel, Aguilera, Antonio, Michelena, Georgina, Aguilar, Cristóbal, Ilyina, Anna
- Food science and biotechnology 2017 v.26 no.4 pp. 993-1001
- Kluyveromyces marxianus, autoclaves, autoclaving, banana peels, cellulose, enzymatic hydrolysis, ethanol, ethanol production, fermentation, grinding, hemicellulose, hydrolysis, lignin, reducing sugars, sugar content, sulfuric acid, temperature
- Pretreatments with different concentrations of sulfuric acid (0, 0.5, and 1% v/v) and temperatures (28 and 121 °C at 103 kPa in an autoclave) were performed on banana peels (BP) milled by mechanical grinding and grinding in a blender as well as without grinding. Cellulose, hemicellulose, lignin, ash, and total and reducing sugar contents were evaluated. The highest yields of cellulose enzymatic hydrolysis (99%) were achieved with liquefied autoclaved BP treated with 0.5 and 1% acid after 48 h of hydrolysis. Ethanol production by Kluyveromyces marxianus fermentation was assayed using hydrolyzed BP at 10, 15, and 20% (w/w). The highest ethanol level (21 g/L) was reached after 24 h of fermentation with 20% (w/w) BP. Kinetics of the consumption of reducing sugars under this fermentation condition demonstrates the presence of a lag period (about 8 h). Thus, BP are a good source for ethanol production.