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Chemical Analysis of Fermentable Sugars and Secondary Products in 23 Sweet Sorghum Cultivars

Author:
Uchimiya, Minori, Knoll, Joseph E., Anderson, William F., Harris-Shultz, Karen R.
Source:
Journal of agricultural and food chemistry 2017 v.65 no.35 pp. 7629-7637
ISSN:
1520-5118
Subject:
Sorghum bicolor, Zea mays, biofuels, biomass, brix, chemical analysis, citrates, corn, cultivars, drought tolerance, electrical conductivity, electrodes, experimental design, factor analysis, females, fermentation, fluorescence, fructose, fuel production, glucose, hybrids, inbred lines, juices, lignocellulose, loamy sand soils, males, market value, maturity stage, nitrogen content, organic carbon, oxalates, pH, phenolic compounds, planting date, spectroscopy, sucrose, sweet sorghum, value-added products
Abstract:
Sorghum (Sorghum bicolor (L.) Moench) is a heat- and drought-tolerant crop that has promise to supplement corn (Zea mays L.) for biofuel production from fermentable sugars (for sweet cultivars) and lignocellulosic biomass. Quantitative relationships are lacking to predict the accumulation of primary (stem sugars) and secondary (organic acids, phenolics, and inorganic species) products that could either expand (as the value-added product) or limit (as the fermentation inhibitor) the market value of a cultivar. Five male (Atlas, Chinese, Dale, Isidomba, N98) and three female (N109B, N110B, and N111B) inbred lines and their hybrids (23 cultivars total) were planted on a Tifton loamy sand in April, May, and June of 2015 in a triplicate split-plot design and were harvested at the hard-dough maturity stage. Stalk juices were analyzed for sugar (glucose, fructose, and sucrose) and organic acid (citrate, oxalate, and cis- and trans-aconitic acid) concentrations, Brix, pH, electric conductivity (EC), total organic carbon (TOC), and total nitrogen (TN), and by fluorescence excitation emission spectrophotometry with parallel factor analysis (EEM/PARAFAC). Later plantings consistently (p < 0.05) (1) increased sucrose, total sugar, and trans-aconitic acid concentrations, Brix, and TOC and (2) decreased EC. Sucrose, total sugar, pH, EC, and Brix showed significant cultivar × planting date interactions. Observed linear relationships (Pearson’s) could be used to deploy simple and inexpensive electrode (EC) and fluorescence-based field methods to predict the primary products from secondary products, and vise versa.
Agid:
5799817