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Absorption Kinetics of the Main Conjugated Linoleic Acid Isomers in Commercial-Rich Oil after Oral Administration in Rats

Author:
Rodríguez-Alcalá, Luís M., Ares, Irma, Fontecha, Javier, Martínez-Larrañaga, María-Rosa, Anadón, Arturo, Martínez, María-Aránzazu
Source:
Journal of agricultural and food chemistry 2017 v.65 no.35 pp. 7680-7686
ISSN:
1520-5118
Subject:
absorption, conjugated linoleic acid, dose response, flame ionization, humans, isomers, oils, oral administration, pharmacokinetics, rats
Abstract:
This study aimed to assess the oral absorption and plasma kinetics of two main isomers contained in commercial conjugated linoleic acid (CLA)-rich oil (Tonalin TG-80), rumenic acid (RA), and C18:2 trans-10, cis-12. The isomer plasma disposition after the single oral dose of 3000 mg of Tonalin TG-80/kg, containing 1200 mg/kg of each isomer, was studied in rats. The isomer plasma concentrations were determined by gas chromatography with flame ionization detection. The plasma kinetics showed rapid oral absorption of RA and C18:2 trans-10, cis-12 (t₁/₂ₐ 0.34 ± 0.09 and 0.53 ± 0.01 h) and slow elimination (t₁/₂ᵦ 25.68 ± 3.29 and 18.12 ± 1.71 h); the maximal isomer plasma concentrations (Cₘₐₓ) of 8.48 ± 0.98 and 7.67 ± 0.80 μg mL–¹, respectively, were estimated at 2.08 ± 0.14 and 2.26 ± 0.11 h. Our results from a preclinical kinetic study in rats help to design future studies in humans for evaluating the CLA isomer dose–response.
Agid:
5799823