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Absorption Kinetics of the Main Conjugated Linoleic Acid Isomers in Commercial-Rich Oil after Oral Administration in Rats

Rodríguez-Alcalá, Luís M., Ares, Irma, Fontecha, Javier, Martínez-Larrañaga, María-Rosa, Anadón, Arturo, Martínez, María-Aránzazu
Journal of agricultural and food chemistry 2017 v.65 no.35 pp. 7680-7686
absorption, conjugated linoleic acid, dose response, flame ionization, humans, isomers, oils, oral administration, pharmacokinetics, rats
This study aimed to assess the oral absorption and plasma kinetics of two main isomers contained in commercial conjugated linoleic acid (CLA)-rich oil (Tonalin TG-80), rumenic acid (RA), and C18:2 trans-10, cis-12. The isomer plasma disposition after the single oral dose of 3000 mg of Tonalin TG-80/kg, containing 1200 mg/kg of each isomer, was studied in rats. The isomer plasma concentrations were determined by gas chromatography with flame ionization detection. The plasma kinetics showed rapid oral absorption of RA and C18:2 trans-10, cis-12 (t₁/₂ₐ 0.34 ± 0.09 and 0.53 ± 0.01 h) and slow elimination (t₁/₂ᵦ 25.68 ± 3.29 and 18.12 ± 1.71 h); the maximal isomer plasma concentrations (Cₘₐₓ) of 8.48 ± 0.98 and 7.67 ± 0.80 μg mL–¹, respectively, were estimated at 2.08 ± 0.14 and 2.26 ± 0.11 h. Our results from a preclinical kinetic study in rats help to design future studies in humans for evaluating the CLA isomer dose–response.