Main content area

Pharmacokinetics of Rhodamine 110 and Its Organ Distribution in Rats

Jiang, Shiau-Han, Cheng, Yung-Yi, Huo, Teh-Ia, Tsai, Tung-Hu
Journal of agricultural and food chemistry 2017 v.65 no.35 pp. 7797-7804
bioavailability, dyes, food additives, high performance liquid chromatography, kidneys, liver, oral administration, pharmacokinetics, rats, tandem mass spectrometry
Rhodamine dyes have been banned as food additives due to their potential tumorigenicity. Rhodamine 110 is illegal as a food additive, although its pharmacokinetics have not been characterized, and no accurate bioanalytical methods are available to quantify rhodamine 110. The aim of this study was to develop and validate a fast, stable, and sensitive method to quantify rhodamine 110 using high-performance liquid chromatography coupled to tandem mass spectrometry (HPLC-MS/MS) to assess its pharmacokinetics and organ distribution in awake rats. Rhodamine 110 exhibited linear pharmacokinetics and slow elimination after oral administration. Furthermore, its oral bioavailability was approximately 34–35%. The distribution in the liver and kidney suggests that these organs are primarily responsible for rhodamine 110 metabolism and elimination. Our investigation describes the pharmacokinetics and a quantification method for rhodamine 110, improving our understanding of the food safety of rhodamine dyes.