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Anxiety-like behavioural effects of extremely low-frequency electromagnetic field in rats

Djordjevic, Natasa Z., Paunović, Milica G., Peulić, Aleksandar S.
Environmental science and pollution research international 2017 v.24 no.27 pp. 21693-21699
adults, bioactive properties, electromagnetic field, hypothalamus, males, nitric oxide, nitrites, oxidative stress, oxygen, rats, superoxide anion
In recent years, extremely low-frequency electromagnetic field (ELF-EMF) has received considerable attention for its potential biological effects. Numerous studies have shown the role of ELF-EMF in behaviour modulation. The aim of this study was to investigate the effect of short-term ELF-EMF (50 Hz) in the development of anxiety-like behaviour in rats through change hypothalamic oxidative stress and NO. Ten adult male rats (Wistar albino) were divided in two groups: control group—without exposure to ELF-EMF and experimental group—exposed to ELF-EMF during 7 days. After the exposure, time open field test and elevated plus maze were used to evaluate the anxiety-like behaviour of rats. Upon completion of the behavioural tests, concentrations of superoxide anion (O₂·⁻), nitrite (NO₂ ⁻, as an indicator of NO) and peroxynitrite (ONOO⁻) were determined in the hypothalamus of the animals. Obtained results show that ELF-EMF both induces anxiety-like behaviour and increases concentrations of O₂·⁻ and NO, whereas it did not effect on ONOO⁻ concentration in hypothalamus of rats. In conclusion, the development of anxiety-like behaviour is mediated by oxidative stress and increased NO concentration in hypothalamus of rats exposed to ELF-EMF during 7 days.