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Characterization and Immunomodulatory Activity of a Novel Peptide, ECFSTA, from Wheat Germ Globulin

Wu, Wenjia, Zhang, Mengmeng, Ren, Yao, Cai, Xinze, Yin, Zhina, Zhang, Xiaoai, Min, Tian, Wu, Hui
Journal of agricultural and food chemistry 2017 v.65 no.27 pp. 5561-5569
Toll-like receptor 2, Toll-like receptor 4, functional foods, gel chromatography, globulins, immunomodulators, interleukin-6, ion exchange chromatography, macrophages, nitric oxide, phagocytosis, reactive oxygen species, reversed-phase high performance liquid chromatography, secretion, tumor necrosis factor-alpha, wheat germ
A novel peptide was extracted from wheat germ globulin and purified using ion-exchange chromatography, gel filtration chromatography, and semi-preparative reversed-phase high-performance liquid chromatography (RP-HPLC). The sequence of the peptide was found to be Glu-Cys-Phe-Ser-Thr-Ala (ECFSTA). Its immunomodulatory effects were evaluated, and the results showed that ECFSTA could enhance phagocytosis of RAW 264.7 cells and significantly increase their secretion of nitric oxide (NO), interleukin 6 (IL-6), tumor necrosis factor α (TNF-α), and reactive oxygen species (ROS). ECFSTA activated macrophages mainly through the pattern recognition receptors (PRRs) of toll-like receptor 2 (TLR2) and toll-like receptor 4 (TLR4), and the production of ROS simultaneously stimulated macrophages to produce TNF-α. Thus, ECFSTA could be used as an immunomodulator and might be a promising component of functional foods.