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Characterization and Immunomodulatory Activity of a Novel Peptide, ECFSTA, from Wheat Germ Globulin
- Wu, Wenjia, Zhang, Mengmeng, Ren, Yao, Cai, Xinze, Yin, Zhina, Zhang, Xiaoai, Min, Tian, Wu, Hui
- Journal of agricultural and food chemistry 2017 v.65 no.27 pp. 5561-5569
- Toll-like receptor 2, Toll-like receptor 4, functional foods, gel chromatography, globulins, immunomodulators, interleukin-6, ion exchange chromatography, macrophages, nitric oxide, phagocytosis, reactive oxygen species, reversed-phase high performance liquid chromatography, secretion, tumor necrosis factor-alpha, wheat germ
- A novel peptide was extracted from wheat germ globulin and purified using ion-exchange chromatography, gel filtration chromatography, and semi-preparative reversed-phase high-performance liquid chromatography (RP-HPLC). The sequence of the peptide was found to be Glu-Cys-Phe-Ser-Thr-Ala (ECFSTA). Its immunomodulatory effects were evaluated, and the results showed that ECFSTA could enhance phagocytosis of RAW 264.7 cells and significantly increase their secretion of nitric oxide (NO), interleukin 6 (IL-6), tumor necrosis factor α (TNF-α), and reactive oxygen species (ROS). ECFSTA activated macrophages mainly through the pattern recognition receptors (PRRs) of toll-like receptor 2 (TLR2) and toll-like receptor 4 (TLR4), and the production of ROS simultaneously stimulated macrophages to produce TNF-α. Thus, ECFSTA could be used as an immunomodulator and might be a promising component of functional foods.