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Plasma Pharmacokinetics, Bioavailability, and Tissue Distribution of Four C-Glycosyl Flavones from Mung Bean (Vigna radiata L.) Seed Extracts in Rat by Ultrahigh-Performance Liquid Chromatography–Tandem Mass Spectrometry

Author:
Bai, Yan, Zhang, Qili, Wang, Baoyu, Zhang, Meiyan, Xu, Yan, Li, Shasha, Zhao, Yunli, Yu, Zhiguo
Source:
Journal of agricultural and food chemistry 2017 v.65 no.27 pp. 5570-5580
ISSN:
0021-8561
Subject:
Vigna radiata, bioactive properties, bioavailability, flavones, liquid chromatography, mung beans, oral administration, pharmacokinetics, polyphenols, rats, seed extracts, tandem mass spectrometry, tissue distribution, tissues
Abstract:
The main polyphenols in mung bean (Vigna radiata L.) seed (MBS), an edible legume with various biological activities, are C-glycosyl flavones (vitexin, isovitexin, isovitexin-6″-O-α-l-glucoside, and dulcinoside). In our study, a validated ultrahigh-performance liquid chromatography–tandem mass spectrometry (UHPLC–MS/MS) method was developed to quantitate the concentrations of four C-glycosyl flavones from MBS extracts in the plasma and various tissues of rats and successfully applied to study their pharmacological profile and tissue distribution in vivo. Four C-glycosyl flavones were rapidly absorbed after oral administration, achieving a Cmax at around 1.5 h, and they could be distributed widely and rapidly in tested tissues. The concentrations of four C-glycosyl flavones in all of the tested tissues decreased obviously in 4 h, which indicated that there was not a trend of long-term accumulation of them. This is the first time to report on pharmacokinetic and tissue distribution studies of four C-glycosyl flavones in rat. The results provided a significative basis for the application of MBS.
Agid:
5801348