U.S. flag

An official website of the United States government

Dot gov

Official websites use .gov
A .gov website belongs to an official government organization in the United States.


Secure .gov websites use HTTPS
A lock ( ) or https:// means you’ve safely connected to the .gov website. Share sensitive information only on official, secure websites.


Main content area

Minimum information about a single amplified genome (MISAG) and a metagenome-assembled genome (MIMAG) of bacteria and archaea.

Robert M. Bowers, Nikos C. Kyrpides, Ramunas Stepanauskas, Miranda Harmon-Smith, Devin Doud, T. Reddy B.K., Frederik Schulz, Jessica Jarett, Adam R. Rivers, Emiley A. Eloe-Fadrosh, Susannah G. Tringe, Natalia N. Ivanova, Alex Copeland, Alicia Clum, Eric D. Becraft, Rex R. Malmstrom, Bruce Birren, Mircea Podar, Peer Bork, George M. Weinstock
Nature Biotechnology 2017 v.35 no.8 pp. 725-731
Archaea, bacteria, biotechnology, genetic markers, genome, information, metagenomics, nucleotide sequences, standards and grades
We present two standards developed by the Genomic Standards Consortium (GSC) for reporting bacterial and archaeal genome sequences. Both are extensions of the minimum information about any (x) sequence (MIxS). The standards are the minimum information about a single amplified genome (MISAG) and the minimum information about a metagenome-assembled genome (MIMAG), including (but not limited to) assembly quality, and estimates of genome completeness and contamination. These standards can be used in combination with other GSC checklists including the minimum information about a genome sequence (MIGS), minimum information about a metagenomic sequence (MIMS) and minimum information about a marker gene sequence (MIMARKS). Community-wide adoption of MISAG and MIMAG will facilitate more robust comparative genomic analyses of bacterial and archaeal diversity.