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Identification of a maize chlorotic dwarf virus silencing suppressor protein

Author:
Stewart, Lucy R., Jarugula, Sridhar, Zhao, Yujing, Qu, Feng, Marty, DeeMarie
Source:
Virology 2017 v.504 pp. 88-95
ISSN:
0042-6822
Subject:
Maize chlorotic dwarf virus, Parsnip yellow fleck virus, RNA interference, Rice tungro spherical virus, chlorosis, genome, polyproteins, proteinases, strains, viruses
Abstract:
Maize chlorotic dwarf virus (MCDV), a member of the genus Waikavirus, family Secoviridae, has a 11784 nt (+)ssRNA genome that encodes a 389 kDa proteolytically processed polyprotein. We show that an N-terminal 78kDa polyprotein (R78) has silencing suppressor activity, that it is cleaved by the viral 3C-like protease into 51 and 27 kDa proteins (p51 and p27), and that the p51 protein has silencing suppressor activity. Silencing suppressor activity of this protein is conserved in three divergent MCDV strains (MCDV-Severe, MCDV-M1, and MCDV-Tennessee) and the recently described waikavirus Bellflower vein chlorosis virus. However, no silencing suppression was detected for the orthologous protein of the waikavirus Rice tungro spherical virus, type strain (RTSV-A) or Parsnip yellow fleck virus (PYFV) from the genus Sequivirus in the same family. This is the first identification of a virus suppressor of RNA silencing for MCDV and for the genus Waikavirus.
Agid:
5801853
Handle:
10113/5801853