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Investigation of the key factors on 3-hydroxypropionic acid production with different recombinant strains
- Niu, Kun, Cheng, Xiu-Li, Qin, Hai-Bin, Liu, Ji-Song, Zheng, Yu-Guo
- 3 Biotech 2017 v.7 no.5 pp. 314
- bacteria, glycerol, glycerol-3-phosphate dehydrogenase, malic acid, polymers
- 3-Hydroxypropionic acid (3-HP) is an important compound and precursor for a series of chemicals and polymeric materials. In this study, the 3-HP producing bacteria were constructed and studied for efficient synthesis of 3-HP. The results indicated that the instability of glycerol dehydratase (GDHt) affected the 3-HP production significantly, which was successfully solved by the expression of glycerol dehydratase reactivase (GdrB), with fivefold increase in 3-HP yield. Meanwhile, NAD⁺-regenerating enzymes GPD1 (glycerol-3-phosphate dehydrogenase) was expressed; however, the results showed 3-HP was significantly decreased from 56.73–4 mM, and malic acid was obviously increased. Analysis of the C flux distribution showed that the main reason for the results was the lack of NAD⁺. The addition of NAD⁺ further increased the 3-HP production to 23.87 mM, demonstrating that the “regeneration of NAD⁺” was the major factor for enhancing 3-HP production.