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Anti-CD25 monoclonal antibody enhances the protective efficacy of Schistosoma japonicum GST vaccine via inhibition of CD4+CD25+Foxp3+ regulatory T cells

Author:
Tang, Chun-lian, Yang, Jin, Cheng, Liang-yu, Cheng, Lan-fang, Liu, Zhi-ming
Source:
Parasitology research 2017 v.116 no.10 pp. 2727-2732
ISSN:
0932-0113
Subject:
Schistosoma japonicum, T-lymphocytes, cercariae, glutathione transferase, immunization, interferon-gamma, interleukin-2, mice, monoclonal antibodies, splenocytes, vaccines
Abstract:
The effect of anti-CD25 monoclonal antibody (anti-CD25 mAb) on the protection efficacy of Schistosoma japonicum 26 kDa GST (glutathione-S-transferase) vaccine was evaluated. Mice were immunized with GST before infection with S. japonicum cercariae and then injected with anti-CD25 mAb. The worm reduction rate was promoted from 24.18% in mice with GST immunization to 47.09% in mice with GST plus anti-CD25 mAb. Compared with the control group, the percentages of splenic CD4⁺CD25⁺Foxp3⁺ regulatory T cells (Tregs) were significantly lower after administration of anti-CD25 mAb; meanwhile, elevated levels of IFN-γ and IL-2 were secreted by splenocytes. These results indicate that the poor protective efficacy of the GST vaccine against S. japonicum results from the presence of CD4⁺CD25⁺Foxp3⁺ Tregs, while anti-CD25 mAb can partially block CD4⁺CD25⁺Foxp3⁺ Tregs and thus enhance the protective efficacy of the GST vaccine.
Agid:
5803302