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Polymorphic Amplified Typing Sequences (PATS) Strain Typing System Accurately Discriminates a Set of Temporally and Spatially Disparate Escherichia coli 0157 Isolates Associated with Human Infection
- Kudva, Indira T., Smole, Sandra, Griffin, Robert W., Garren, Jeonifer, Kalia, Nimisha, Murray, Megan, John, Manohar, Timperi, Ralph, Calderwood, Stephen B.
- The Open Microbiology Journal 2013 v.7 pp. 123
- Escherichia coli O157, human diseases, polymerase chain reaction, pulsed-field gel electrophoresis
- Polymorphic Amplified Typing Sequences (PATS) is a PCR-based Escherichia coli O157 (O157) strain typing system. Here, we show that PATS compares excellently with Pulsed-Field Gel Electrophoresis (PFGE) in that both methods cluster geographically diverse O157 isolates similarly. Comparative analysis of the results obtained in this simulated “blind” study attests to the discriminating power and applicability of PATS to epidemiological/nosocomial situations.