PubAg

Main content area

Polymorphic Amplified Typing Sequences (PATS) Strain Typing System Accurately Discriminates a Set of Temporally and Spatially Disparate Escherichia coli 0157 Isolates Associated with Human Infection

Author:
Kudva, Indira T., Smole, Sandra, Griffin, Robert W., Garren, Jeonifer, Kalia, Nimisha, Murray, Megan, John, Manohar, Timperi, Ralph, Calderwood, Stephen B.
Source:
The Open Microbiology Journal 2013 v.7 pp. 123
ISSN:
1874-2858
Subject:
Escherichia coli O157, human diseases, polymerase chain reaction, pulsed-field gel electrophoresis
Abstract:
Polymorphic Amplified Typing Sequences (PATS) is a PCR-based Escherichia coli O157 (O157) strain typing system. Here, we show that PATS compares excellently with Pulsed-Field Gel Electrophoresis (PFGE) in that both methods cluster geographically diverse O157 isolates similarly. Comparative analysis of the results obtained in this simulated “blind” study attests to the discriminating power and applicability of PATS to epidemiological/nosocomial situations.
Agid:
58034
Handle:
10113/58034