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Antibacterial and antioxidant properties of grape stem extract applied as disinfectant in fresh leafy vegetables

Author:
Vázquez-Armenta, F. J., Silva-Espinoza, B. A., Cruz-Valenzuela, M. R., González-Aguilar, G. A., Nazzaro, F., Fratianni, F., Ayala-Zavala, J. F.
Source:
Journal of food science and technology 2017 v.54 no.10 pp. 3192-3200
ISSN:
0022-1155
Subject:
2,2-diphenyl-1-picrylhydrazyl, Escherichia coli O157, Listeria monocytogenes, Staphylococcus aureus, Vitis vinifera, animal pathogenic bacteria, antimicrobial properties, antioxidant activity, bioactive compounds, byproducts, caffeic acid, catechin, chlorogenic acid, disinfectants, ferulic acid, food pathogens, gallic acid, grapes, lettuce, microbial growth, rutin, sanitizers, spinach
Abstract:
In the present study total phenolic content (TPC), total flavonoid content (TFC), antioxidant activity and antimicrobial properties of grape (Vitis vinifera var. Red Globe) stem extract is reported. Also, the identification of main phenolic compounds was carried out by UPLC-PAD analysis. TPC and TFC of extract were 37.25 g GAE kg⁻¹ and 98.07 g QE kg⁻¹, respectively. Extract showed an antioxidant capacity of 132.60 and 317 g TE kg⁻¹ for DPPH and ABTS radical scavenging capacity, respectively. The main phenolic compounds identified were rutin, gallic acid, chlorogenic acid, caffeic acid, catechin and ferulic acid. Extract inhibited the growth of Listeria monocytogenes, Staphylococcus aureus, Salmonella enterica subsp. enterica serovar Typhimurium, and Escherichia coli O157: H7 at MIC range 16–18 g L⁻¹. Extract affected the different phases of bacterial growth. In addition, application of Extract (25 g L⁻¹) as a sanitizer was effective to reduce the populations of all bacteria inoculated in lettuce (0.859–1.884 log reduction) and spinach (0.843–2.605 log reduction). This study emphasizes the potential of grape processing byproducts as an emergent and attractive source of bioactive compounds with antioxidant properties and antimicrobial activity against important foodborne pathogens. The study demonstrated that stem extract could be used to control the presence of human pathogenic bacteria in fresh leafy vegetables.
Agid:
5803957