U.S. flag

An official website of the United States government

Dot gov

Official websites use .gov
A .gov website belongs to an official government organization in the United States.


Secure .gov websites use HTTPS
A lock ( ) or https:// means you’ve safely connected to the .gov website. Share sensitive information only on official, secure websites.


Main content area

Effects of voluntary running and soy supplementation on diet-induced metabolic disturbances and inflammation in mice

Lin Yan, George L. Graef, Kate J. Claycombe, LuAnn K. Johnson
Journal of agricultural and food chemistry 2013 v.61 no.39 pp. 9373-9379
adiponectin, body fat, casein, chemokines, dietary supplements, enzyme inhibitors, exercise, food intake, glucose, high fat diet, inflammation, insulin, leptin, mice, obesity, plasminogen activator, running (physical activity), soy protein isolate, triacylglycerols, tumor necrosis factor-alpha, weight control, weight gain, weight loss
The present study investigated the effects of voluntary running and soy supplementation on diet-induced metabolic disturbance and inflammation in male C57BL/6 mice using a 2x2x2 design in which the effects of diet (AIN93G or its modification with 45% calories from fat), activity level (sedentary or running) and protein source (casein or soy protein isolate (SPI)) and their interactions were assessed. The high-fat diet significantly increased whereas running significantly reduced body weight gain and fat mass compared to their respective AIN93G and sedentary controls; the SPI supplementation did not affect either measurements compared to the casein-based diet. The high-fat diet significantly increased plasma concentrations of insulin, glucose, triglycerides, leptin, monocyte chemotactic protein-1 (MCP-1), plasminogen activator inhibitor-1 (PAI-1) and tumor necrosis factor-a (TNF-a), and running significantly reduced these parameters. The high-fat diet significantly reduced plasma adiponectin concentration while running significantly increased plasma adiponectin. The SPI feeding significantly reduced plasma levels of insulin, glucose, triglycerides, leptin, MCP-1 and TNF-a (but it did not affect PAI-1 and adiponectin); particularly it significantly reduced plasma insulin, MCP-1 and TNF-a in high-fat diet-fed groups. These results indicate that both voluntary running and SPI supplementation down-regulates inflammation in high-fat diet-fed mice. The difference between them was that the former, but not the latter, was through an action of weight reduction. Further investigations are warranted to the roles of soy protein as a dietary modification in weight management and in reducing the risk of obesity, particularly to those with restricted capability to be physically active.