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Comparative proteomic analysis and characterization of benzo(a)pyrene removal by Microbacterium sp. strain M.CSW3 under denitrifying conditions

Qin, Wei, Fan, FuQiang, Zhu, Yi, Wang, Yingying, Liu, Xiang, Ding, Aizhong, Dou, Junfeng
Bioprocess and biosystems engineering 2017 v.40 no.12 pp. 1825-1838
Microbacterium, benzo(a)pyrene, biochemical pathways, bioremediation, carbon, catalytic activity, denitrification, energy, gene expression regulation, glucose, heavy metals, human health, metal ions, organic acids and salts, pH, persistent organic pollutants, phenanthrene, polycyclic aromatic hydrocarbons, protein synthesis, proteins, proteomics, risk, soil
High-molecular-weight polycyclic aromatic hydrocarbons are persistent organic pollutants with great environmental and human health risks and the associated bioremediation activities have always been hampered by the lack of powerful bacterial species under redox conditions. A Microbacterium sp. strain capable of using benzo(a)pyrene as sole carbon and energy sources under denitrifying conditions was isolated. The difference in protein expression during BaP removal and removal characterization were investigated. A total of 146 proteins were differentially expressed, 44 proteins were significantly up-regulated and 102 proteins were markedly down-regulated. GO and COG analysis showed that BaP removal inhibited the expression of proteins related to glucose metabolism at different levels and activated other metabolic pathway. The proteins associated with catalytic activity and metabolic process were altered significantly. Furthermore, the BaP removal might be occurred in certain organelle of M.CSW3. The strain removed BaP with a speed of 0.0657–1.0072 mg/L/day over the concentrations range 2.5–100 mg/L. High removal rates (>70%) were obtained over the range of pH 7–11 in 14 days. Carbohydrates and organic acids which could be utilized by the strain, as well as heavy metal ions, reduced BaP removal efficiency. However, phenanthrene or pyrene addition enhanced the removal capability of M.CSW3. The strain was proved to have practical potential for bioremediation of PAHs-contaminated soil and this study provided a powerful platform for further application by improving production of associated proteins.