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A homofermentative Bacillus sp. BC-001 and its performance as a potential L-lactate industrial strain

Author:
Thitiprasert, Sitanan, Kodama, Kentaro, Tanasupawat, Somboon, Prasitchoke, Phatthanon, Rampai, Tanapawarin, Prasirtsak, Budsabathip, Tolieng, Vasana, Piluk, Jiraporn, Assabumrungrat, Suttichai, Thongchul, Nuttha
Source:
Bioprocess and biosystems engineering 2017 v.40 no.12 pp. 1787-1799
ISSN:
1615-7591
Subject:
Bacillus (bacteria), calcium, fermentation, fermenters, glucose, heat tolerance, lactic acid, pH, saccharification, starch, sucrose
Abstract:
Bacillus sp. BC-001 was first reported as a potent thermotolerant and homofermentative strain for an industrial-scale L-lactate production. In a flask culture, this isolate fermented both glucose and sucrose to lactate with high yield (0.96 and 0.87 g/g) and productivity (2.8 and 2.6 g/L h), respectively. The higher lactate production performance was obtained in the simultaneous saccharification and fermentation of liquefied starch (150.1 g/L final titer, 0.98 g/g yield, 3.2 g/L h productivity) and the fed-batch glucose fermentation (139.9 g/L final titer, 0.96 g/g yield, 2.9 g/L h productivity). Significant increase in lactate productivity (5.5–6.1 g/L h) was obtained from the high/heavy-inoculum seed in the stirred tank fermentor. Both calcium bases and monovalent bases were successfully employed for pH control during lactate fermentation by this isolate resulting in a versatile and simple operation. By the two-phase fermentation using the high/heavy-inoculum seed of BC-001, the fermentation reproducibility was acquired toward the pilot-scale fermentors.
Agid:
5806787