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Fabrication of Stable and Luminescent Copper Nanocluster-Based AIE Particles and Their Application in β-Galactosidase Activity Assay

Zhao, Meizhi, Qian, Zhaosheng, Zhong, Mengting, Chen, Zhentian, Ao, Hang, Feng, Hui
ACS applied materials & interfaces 2017 v.9 no.38 pp. 32887-32895
beta-galactosidase, copper, electron transfer, enzyme activity, hydrophobic bonding, hydrophobicity, ligands, luminescence, monitoring, nanoparticles, p-nitrophenol
Thiolated copper nanoclusters (CuNCs) with aggregation-induced emission characteristic are becoming a novel luminescent material, but it is still a challenging task to retain its bright luminescence in a neutral solution. In this work, we report a new copper nanocluster with aggregation-induced emission (AIE) enhancement property using a hydrophobic molecule as the protecting ligand, and brightly luminescent AIE particles of copper nanocluster were prepared via hydrophobic interaction. These CuNCs AIE particles possess uniform rod-like shapes, with sizes in hundreds of nanometer, and an intense luminescence; more importantly, its luminescence remains stable in neutral and alkaline solutions. It is found that 4-nitrophenol is able to effectively quench the luminescence of CuNC AIE particles through strong hydrophobic interaction and electron transfer between them. This strong quenching effect was adopted to develop a luminescent assay for β-galactosidase at physiological condition. This work presents a demonstration of preparing CuNC AIE particles with bright luminescence at neutral condition and gives an example of the use of AIE particles in monitoring the enzyme activity.