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Tocopherol levels in different mango varieties correlate with MiHPPD expression and its over-expression elevates tocopherols in transgenic Arabidopsis and tomato
- Singh, Rajesh K., Chaurasia, Akhilesh K., Bari, Rupesh, Sane, Vidhu A.
- 3 Biotech 2017 v.7 no.5 pp. 352
- Arabidopsis thaliana, Mangifera indica, Solanum lycopersicum var. lycopersicum, alpha-tocopherol, biosynthesis, ethylene, gene expression, gene expression regulation, genes, heterologous gene expression, mangoes, metabolic engineering, ripening, tomatoes, transgenic plants
- Mango fruit tocopherol levels vary in different varieties during ripening. This study shows that tocopherol accumulation is highly correlated with its p-hydroxyphenyl pyruvate dioxygenase (MiHPPD) gene expression during ripening. MiHPPD transcript is ethylene induced and differentially expressed in four mango varieties used in this study. Higher/lower accumulation of tocopherol (mainly α-tocopherol) was achieved by heterologous expression of MiHPPD in Arabidopsis and tomato. The results suggest that tocopherol accumulation in mango fruit is correlated to MiHPPD gene expression. Over-expression of MiHPPD gene channelizes the flux towards tocophreol biosynthesis and could be used as a potential tool for metabolic engineering.