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Spatial organization of fibroblast and spermatocyte nuclei with different B-chromosome content in Korean field mouse, Apodemus peninsulae (Rodentia, Muridae)

Author:
Karamysheva, Tatyana V., Torgasheva, Anna A., Yefremov, Yaroslav R., Bogomolov, Anton G., Liehr, Thomas, Borodin, Pavel M., Rubtsov, Nikolay B.
Source:
Genome 2017 v.60 no.10 pp. 815-824
ISSN:
1480-3321
Subject:
Apodemus, B chromosomes, centromeres, fibroblasts, fluorescence in situ hybridization, heterochromatin, interphase, mice, microscopy, models
Abstract:
Korean field mouse (Apodemus peninsulae) shows a wide variation in the number of B chromosomes composed of constitutive heterochromatin. For this reason, it provides a good model to study the influence of the number of centromeres and amount of heterochromatin on spatial organization of interphase nuclei. We analyzed the three-dimensional organization of fibroblast and spermatocyte nuclei of the field mice carrying a different number of B chromosomes using laser scanning microscopy and 3D fluorescence in situ hybridization. We detected a co-localization of the B chromosomes with constitutive heterochromatin of the chromosomes of the basic set. We showed a non-random distribution of B chromosomes in the spermatocyte nuclei. Unpaired B chromosomes showed a tendency to occur in the compartment formed by the unpaired part of the XY bivalent.
Agid:
5808419