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The roles of NF-κB and ROS in regulation of pro-inflammatory mediators of inflammation induction in LPS-stimulated zebrafish embryos
- Ko, Eun-Yi, Cho, Su-Hyeon, Kwon, Seung-Hae, Eom, Chi-Yong, Jeong, Myeong Seon, Lee, WonWoo, Kim, Seo-Young, Heo, Soo-Jin, Ahn, Ginnae, Lee, Kang Pa, Jeon, You-Jin, Kim, Kil-Nam
- Fish & shellfish immunology 2017 v.68 pp. 525-529
- Danio rerio, acetylcysteine, gene expression, inducible nitric oxide synthase, inflammation, lipopolysaccharides, messenger RNA, nitric oxide, transcription factor NF-kappa B
- In this study, the roles of reactive oxygen species (ROS) and NF-κB on inflammation induction in lipopolysaccharide (LPS)-stimulated zebrafish embryos were evaluated using N-acetyl-l-cysteine (NAC) and pyrrolidine dithiocarbamate (PDTC), specific inhibitors of ROS and NF-κB, respectively. LPS-stimulated zebrafish embryos showed increasing production of NO and ROS and expression of iNOS and COX-2 protein, compared to a control group without LPS. However, NAC significantly inhibited production of NO and ROS and markedly suppressed expression of iNOS and COX-2 protein in LPS-stimulated zebrafish embryos. The mRNA expressions of NF-κB such as p65NF-κB and IκB-A were significantly increased after LPS stimulation, whereas PDTC attenuated mRNA expression of NF-κB. PDTC also inhibited production of NO and reduced expression of iNOS and COX-2 protein in LPS-stimulated zebrafish embryos. Taken together, these results indicated that LPS increases pro-inflammatory mediators in zebrafish embryos through ROS and NF-κB regulation.