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Expression of the hepatitis B surface antigen (HbsAg) under the control of mMT-I promoter in transgenic mice can be induced by zinc sulphate, dexamethasone and lipopolysaccharide

Author:
Anastassiadis, C., Rottmann, O., Hörmansdorfer, S., Pirchner, F.
Source:
Journal of animal breeding and genetics 1997 v.114 no.1-6 pp. 225-230
ISSN:
0931-2668
Subject:
Hepatitis B virus, antigens, dexamethasone, females, genes, glucocorticoids, heavy metals, hepatitis B, lipopolysaccharides, males, metallothionein, mice, toxins, zinc sulfate
Abstract:
SUMMARY: Metallothionein genes can be induced in vivo by heavy metals, glucocorticoids, and toxins. In all transgenic mice carrying the MT-I promoter, that have been reported so far, induction by glucocorticoids failed. This study reports two mouse lines, transgenic for the murine MT-I-HBV (hepatitis B virus; map position site: 30-1986) construct, which secrete the viral surface antigen (HBsAg) in their serum. In both lines, males produce more HBsAg than females, and in all cases the MT-I promoter can be induced by dexamethasone, lipopolysaccharide (LPS), and heavy metals. A glucocorticoid-responsive element, which is situated in the HBV fragment used, can explain the dexamethasone induction of the MT-I promoter.
Agid:
581355