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Adsorption effects of the doping relevant peptides Insulin Lispro, Synachten, TB-500 and GHRP 5

Author:
Judák, Péter, Van Eenoo, Peter, Deventer, Koen
Source:
Analytical biochemistry 2017 v.537 pp. 69-71
ISSN:
0003-2697
Subject:
adsorption, insulin, models, peptides, quantitative analysis
Abstract:
The tendency of peptides to adsorb to surfaces can raise a concern in variety of analytical fields where the qualitative/quantitative measurement of low concentration analytes (ng/mL-pg/mL) is required. To demonstrate the importance of using the optimal glassware/plasticware, four doping relevant model peptides (GHRP 5, TB-500, Insulin Lispro, Synachten) were chosen and their recovery from various surfaces were evaluated. Our experiments showed that choosing expensive consumables with low-bind characteristics is not beneficial in all cases. A careful selection of the consumables based on the evaluation of the physico/chemical features of the peptide is recommended.
Agid:
5813729