PubAg

Main content area

Development of a porcine epidemic diarrhea virus M protein-based ELISA for virus detection

Author:
Ren, Xiaofeng, Suo, Siqingaowa, Jang, Yong-Suk
Source:
Biotechnology letters 2011 v.33 no.2 pp. 215-220
ISSN:
0141-5492
Subject:
rabbits, Suid alphaherpesvirus 1, Porcine reproductive and respiratory syndrome virus, Classical swine fever virus, enzyme-linked immunosorbent assay, swine, viruses, genes, Infectious bronchitis virus, Porcine epidemic diarrhea virus, Transmissible gastroenteritis virus, fluorescent antibody technique, Escherichia coli
Abstract:
A membrane (M), protein-based ELISA was developed to detect porcine epidemic diarrhea virus (PEDV). The M gene of PEDV was expressed in Escherichia coli. The purified recombinant M protein was used to immunize rabbits to generate a polyclonal antibody. Immunofluorescence analysis indicated that the anti-PEDV-M antibody reacted with PEDV-infected cells. The antibody was utilized to develop an indirect ELISA to detect PEDV. Other viruses, porcine transmissible gastroenteritis coronavirus, avian infectious bronchitis coronavirus, porcine reproductive and respiratory syndrome virus, classic swine fever virus and porcine pseudorabies virus, were unreactive.
Agid:
581402