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Neutralization of viral infectivity by zebrafish c-reactive protein isoforms
- Bello-Perez, Melissa, Falco, Alberto, Medina-Gali, Regla, Pereiro, Patricia, Encinar, Jose Antonio, Novoa, Beatriz, Perez, Luis, Coll, Julio
- Molecular Immunology 2017 v.91 pp. 145-155
- C-reactive protein, Danio rerio, adaptive immunity, antiviral properties, bacterial infections, biomarkers, coronary disease, eggs, genes, inflammation, microarray technology, mutants, neutralization, pathogenicity, protein isoforms, proteomics, viremia, viruses
- This work explores the unexpected in vivo and in vitro anti-viral functions of the seven c-reactive protein (crp1-7) genes of zebrafish (Danio rerio). First results showed heterogeneous crp1-7 transcript levels in healthy wild-type zebrafish tissues and organs and how those levels heterogeneously changed not only after bacterial but also after viral infections, including those in adaptive immunity-deficient rag1−/− mutants. As shown by microarray hybridization and proteomic techniques, crp2/CRP2 and crp5/CRP5 transcripts/proteins were among the most modulated during in vivo viral infection situations including the highest responses in the absence of adaptive immunity. In contrast crp1/CRP1/and crp7/CRP7 very often remained unmodulated. All evidences suggested that zebrafish crp2-6/CRP2-6 may have in vivo anti-viral activities in addition to their well known anti-bacterial and/or physiological functions in mammalians. Confirming those expectations, in vitro neutralization and in vivo protection against spring viremia carp virus (SVCV) infections were demonstrated by crp2-6/CRP2-6 using crp1-7 transfected and/or CRP1-7-enriched supernatant-treated fish cells and crp2-5-injected one-cell stage embryo eggs, respectively. All these findings discovered a crp1-7/CRP1-7 primitive anti-viral functional diversity.These findings may help to study similar functions on the one-gene-coded human CRP, which is widely used as a clinical biomarker for bacterial infections, tissue inflammation and coronary heart diseases.