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Effect of pseudolaric acid B on biochemical and physiologic characteristics in Colletotrichum gloeosporioides

Author:
Zhang, Jing, Han, Ru-Yue, Ye, Huo-Chun, Zhou, Ying, Zhang, Zheng-Ke, Yuan, En-Lin, Li, Ye, Yan, Chao, Liu, Xia, Feng, Gang, Guo, Yong-Xia
Source:
Pesticide biochemistry and physiology 2018 v.147 pp. 75-82
ISSN:
0048-3575
Subject:
Glomerella cingulata, Pseudolarix amabilis, anthracnose, antifungal properties, biosynthesis, cell walls, ergosterol, growth retardation, mangoes, median effective concentration, membrane permeability, mycelium, plant pathogenic fungi, reducing sugars, spore germination, ultrastructure
Abstract:
In our previous study on natural products with fungicidal activity, pseudolaric acid B (PAB) isolated from Pseudolarix amabilis was examined to inhibit significantly mango anthracnose (Colletotrichum gloeosporioides) in vivo and in vitro. In the current study, sensitivity of 17 plant pathogenic fungi to PAB was determined. Mycelial growth rate results showed that PAB possessed strong antifungal activities to eleven fungi with median effective concentration (EC50) values ranging from 0.087 to 1.927μg/mL. EC50 of PAB against spore germination was greater than that of mycelium growth inhibition, which suggest that PAB could execute antifungal activity through mycelial growth inhibition. Further action mechanism of PAB against C. gloeosporioides was investigated, in which PAB treatment inhibited mycelia dry weight, decreased the mycelia reducing sugar and soluble protein. Furthermore, PAB induced an increase in membrane permeability, inhibited the biosynthesis of ergosterol, caused the extreme alteration in ultrastructure as indicated by the thickened cell wall and increased vesicles. These results will increase our understanding of action mechanism of PAB against plant pathogenic fungi.
Agid:
5815663