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Strain-specific association of soybean dwarf virus small subgenomic RNA with virus particles
- Thanuja Thekke-Veetil, Nancy K. McCoppin, Leslie L. Domier
- Virus research 2017 pp. -
- Aphidoidea, Nicotiana benthamiana, RNA, Soybean dwarf virus, Trifolium pratense, clones, coat proteins, leaves, mutants, open reading frames, plant diseases and disorders, plant viruses, ribonucleases, soybeans, sucrose, virion
- Soybean dwarf virus (SbDV) produces a large subgenomic RNA (LsgRNA) for expression of structural and movement proteins and a small subgenomic RNA (SsgRNA) that does not contain an open reading frame. Sucrose gradient-purified SbDV virions from soybean plants systemically infected with SbDV by aphids and Nicotiana benthamiana leaves agroinfiltrated with infectious clones of two red clover SbDV isolates encapsidated genomic RNA and were associated with SsgRNA in a strain-specific manner. The LsgRNA was protected from RNase degradation, but not packaged into virions as indicated by its presence primarily in ELISA-negative fractions near the tops of sucrose gradients even in mutants that did not express coat protein. Nucleotide differences in the SsgRNA region between isolates conferred differential association of SsgRNA with virions.