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Peptides derived from in vitro gastrointestinal digestion of germinated soybean proteins inhibit human colon cancer cells proliferation and inflammation
- González-Montoya, Marcela, Hernández-Ledesma, Blanca, Silván, Jose Manuel, Mora-Escobedo, Rosalva, Martínez-Villaluenga, Cristina
- Food chemistry 2018 v.242 pp. 75-82
- anti-inflammatory activity, beta-conglycinin, chromatography, colorectal neoplasms, cytotoxicity, digestion, functional foods, gastrointestinal system, germination, glutamine, glycinin, humans, in vitro digestion, inflammation, lipopolysaccharides, macrophages, neoplasm cells, pancreatin, pepsin, peptides, protein concentrates, soy protein, soybeans, ultrafiltration
- The aim was to investigate the potential of germinated soybean proteins asa source of peptides with anticancer and anti-inflammatory activities produced after simulated gastrointestinal digestion. Protein concentrate from germinated soybean was hydrolysed with pepsin/pancreatin and fractionated by ultrafiltration. Whole digest and fractions>10, 5–10, and<5kDa caused cytotoxicity to Caco-2, HT-29, HCT-116 human colon cancer cells, and reduced inflammatory response caused by lipopolysaccharide in macrophages RAW 264.7. Antiproliferative and anti-inflammatory effects were generally higher in 5–10kDa fractions. This fraction was further purified by semi-preparative chromatography and characterised by HPLC-MS/MS. The most potent fraction was mainly composed of β-conglycinin and glycinin fragments rich in glutamine. This is the first report on the anti-cancer and anti-inflammatory effects of newly isolated and identified peptides from germinated soybean released during gastrointestinal digestion. These findings highlight the potential of germination as a process to obtain functional foods or nutraceuticals for colon cancer prevention.