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Evaluation of folate-binding proteins and stability of folates in plant foliages

Puthusseri, Bijesh, Divya, Peethambaran, Veeresh, Lokesh, Kumar, Gyanendra, Neelwarne, Bhagyalakshmi
Food chemistry 2018 v.242 pp. 555-559
Arabidopsis, Coriandrum sativum, crops, folate-binding proteins, folic acid, gene expression regulation, genes, leaves, metabolic engineering, microarray technology, quantitative polymerase chain reaction, salicylic acid
The present study reports the presence of folate binding proteins (FBPs) in the plants, coriander and Arabidopsis, and their contributions toward folate enhancement. After observing that salicylic acid (SA) enhanced the accumulation of folates in coriander, a study was conducted in Arabidopsis, where twofold increase in folates occurred in foliage upon SA treatment. For obtaining insights into genes involved in SA-induced folate accumulation, microarray data of responsive genes in Arabidopsis were screened. Based on the expression profiles, 19 genes were further analysed by qPCR. The results revealed that folate biosynthetic genes were largely down-regulated, whereas a gene of a putative folate-binding protein (FPB) was up-regulated, which correlated with a significant increase of FPBs in foliage. This new information on a plant FBP appears useful for metabolic engineering of a wide range of crops to enhance the content and stability of the folates during post-harvest storage.