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Cultivation of Chlorella vulgaris in a pilot-scale photobioreactor using real centrate wastewater with waste glycerol for improving microalgae biomass production and wastewater nutrients removal
- Ren, Hongyan, Tuo, Jinhua, Addy, Min M., Zhang, Renchuan, Lu, Qian, Anderson, Erik, Chen, Paul, Ruan, Roger
- Bioresource technology 2017 v.245 pp. 1130-1138
- Chlorella vulgaris, algae culture, ammonium nitrogen, bacteria, biomass production, carbon nitrogen ratio, chemical oxygen demand, glycerol, lipid content, microalgae, nutrients, phosphorus, photobioreactors, wastewater
- To improve nutrients removal from real centrate wastewater and enhance the microalgae biomass production, cultivation of Chlorella vulgaris in lab and a pilot-scale photobioreactor with waste glycerol was studied. The results showed the optimal concentration of the crude glycerol was 1.0gL−1 with the maximum biomass productivity of 460mgL−1d−1 TVS, the maximum lipid content of 27%, the nutrient removal efficiency of all above 86%, due to more balanced C/N ratio. The synergistic relationship between the wastewater-borne bacteria and the microalgae had significant good influence on nutrient removal. In pilot-scale wastewater-based algae cultivation, with 1gL−1 waste glycerol addition, the average biomass production of 16.7gm−2d−1, lipid content of 23.6%, and the removal of 2.4gm−2d−1 NH4+-N, 2.7gm−2d−1 total nitrogen, 3.0gm−2d−1 total phosphorous, and 103.0gm−2d−1 of COD were attained for 34days semi-continuous mode.