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A polycation coated liposome as efficient siRNA carrier to overcome multidrug resistance B Biointerfaces

Xia, Yuqiong, Wang, Xiaofei, Cheng, He, Fang, Mei, Ning, Pengbo, Zhou, Yulu, Chen, Wei, Song, Hongjin
Colloids and surfaces 2017 v.159 pp. 427-436
cations, chlorides, colloids, doxorubicin, drug therapy, gene silencing, genes, multiple drug resistance, neoplasms, plasmids, small interfering RNA, toxicity, transfection
Multidrug resistance (MDR) is one of the important factors that impede effective chemotherapy against cancer. Codelivery of MDR1 siRNA (silencing ABCB1 gene) and anticancer drug can greatly inhibit tumor proliferation. Here in this work, we synthesized poly(diallyldimethylammonium chloride) (PDADMAC) coated liposome formula as siMDR1 carrier (AL-PDAD-RNA) and applied it to reverse doxorubicin resistance of OVCAR8/ADR cells. The AL-PDAD-RNA can load siRNA effectively and release siRNA under physiological conditions, leading to improved tumor inhibition than free DOX without siRNA treatment. Meanwhile, the gene silencing effect of AL-PDAD-RNA was shown to be comparable to that of commercial transfection agent lipofectamine, but with less toxicity. The main novelty of this work is to offer a new type of siRNA carrier (PDADMAC coated liposome, AL-PDAD), which is simple-structured, highly-effective and non-toxic. Therefore, we anticipate that PDADMAC-coated liposomes would be very promising in the application of other siRNA delivery or even plasmid delivery.